382 Special Procedures II (chap. 23) 



transfer it from 70% alcohol or water into a mixture of 10-15% glycerol 

 in alcohol or water. Leave the dish uncovered imtil most of the alcohol 

 or water has evaporated. Mount in glycerol jelly. Sections are mounted 

 without cover glass support. Thicker specimens probably will require 

 a supported cover glass. With a turntable spin a ring of gold size on the 

 slide. Allow it to dry. If it is not high enough, add more layers to the 

 height desired. 



Melt glycerol jelly in water bath or in oven. Add a drop or two of 

 jelly onto specimen inside ring. Use just enough to fill ring. Warm cover 

 glass and ease horizontally into place. Carefully wipe off any glycerol 

 jelly that works out from cover glass. After a few tries, it becomes rela- 

 tively easy to estimate the correct amoinit of jelly to make a clean 

 mount. Seal cover glass, supported or unsupported, with gold size or 

 other cover glass sealer (page 123). 



Hydra, Embryos, Flukes (stained ivJiole mounts) 



fixation: Many fixatives are suitable: Carnoy's or Gilson for worms, 

 Bouin's or Zenker's for embryos, formol acetic or saturated HgClo 

 acetic (95/5). But before fixing consult the previous section on in- 

 vertebrates or an authoritative source concerning the problem at 

 hand. 



Follow by proper washing and extraction of any undesirable pig- 

 ments or crystals. 



staining: Choice of stain also depends upon type of material. A few of 

 wide usage will be included here. 



Grenacher's Borax Carmine (galigher, 1934) 

 solution: 



carmine, C.l. 75470 3.0 gm. 



borax 4.0 gm. 



distilled water 100.0 ml. 



Boil approximately 30 minutes or until carmine is dissolved. Mix- 

 ture may be allowed to stand until this occurs. Add: 



707o ethyl alcohol 100.0 ml. 



Allow to stand 1-2 days. Filter. 



procedure: (Hydra, flukes, tapeworms, small Crustacea, etc.) 



1. Transfer from 50% alcohol to borax carmine: 3-4 hours or over- 

 night. 



