JV/iole Mounis 38.'^ 



2. Add concentrated HCl, slowly, drop at a time, stirring, until car- 

 mine has precipitated and is brick red. Let stand 6-8 hours or 

 overnight. 



3. Add equal volume of 3% HCl in 70% alcohol and thoroughly 

 mix. Let stand 2-3 minutes until specimens have settled. Draw off 

 precipitated carmine with pipette. Add more acid alcohol, mix, 

 allow to settle and draw off fluid. Repeat until most of carmine is 

 removed. 



4. Add fresh acid alcohol and allow to destain, checking at intervals 

 under microscope: may require 2 hours or more. If exceedingly 

 slow, increase percentage of acid in alcohol. 



5. When destained, replace with 80% alcohol, several changes to re- 

 move acid: over a period of 1 hour. 



6. Dehydrate, 90% alcohol: 30 minutes or more, depending on size 

 of specimen; absolute alcohol: 30 minutes or more. 



7. Clear. For delicate objects this probably should be done gradually 

 throuoh the following concentration of absolute alcohol and ere- 

 osote (or aniline or carbol) xylene; and into pure creosote-xylene. 



8. Mount. For exceedingly delicate objects and roimd worms, heed 

 the warning above concerning slow impregnation with the motmt- 

 ing meditim. Judgment concerning its use rests with experience, 

 biU slowness is invariably necessary for round \vorms. 



Mayer's Carmalum (cowdry, 1952) 



solutions: 



Stock solution: 



carminic acid (C.P. carmine) C.I. 75470 LO gm. 



ammonium alum 10.0 gm. 



distilled water 200.0 ml. 



When dissolved, filter. Add: 



formalin 1.0 ml. 



