Anitnal Parasites 391 



solution: 



Carmine stock solution: 



carmine, C.I. 75470 2.0 gm. 



potassium carbonate 1.0 gm. 



potassium chloride 5.0 gm. 



distilled water 60.0 ml. 



Boil gently for about 5 miniues, color should deepen. 



Working solution: 



carmine stock solution 20.0 ml. 



ammonium hydroxide 30.0 ml. 



methyl alcohol 30.0 ml. 



procedure: 



1. Hydrate slides to water, removing HgCl2. 



2. Stain in any preferred hematoxylin. 



3. Wash in running water: 5 minutes. 



4. Stain in carmine soltition: 5 minutes. 



5. Differentiate: 5 minutes, in: 



absolute ethyl alcohol 80.0 ml. 



methyl alcohol 40.0 ml. 



distilled water 100.0 ml. 



Change solution if it becomes highly colored. 



6. Dehydrate, clear, and motuit. 



results: 



vegetative amoebae — highly stained with carmine, brilliant red; no 

 cell in intestinal wall takes up carmine 



comments: 



Tomlinson and Grocott [1944) describe a phloxine-toltiidine blue 

 stain for malaria. Leishmanial microfilaria, and intestinal protozoa in 

 tissue. 



