Chapter 24 



Special Procedures III 



Special Mounts 



Sections Mounted on Film. Pickett and Sommer {I960) 



Pickett and Sommer use film in place of glass slides for supporting 

 tissue sections. The flexibility of film and the low cost of material and 

 time can be advantageous. Many sections mounted on a long strip of 

 film are stained in one process by using a developing reel and then are 

 plastic-sprayed as a unit instead of having to be individually cover- 

 slipped. A length of film can be stored in a small space, and single sec- 

 tions or groups of sections can be clipped or stapled to records or re- 

 ports, and filed or shipped in envelopes. Film with section also can be 

 mounted in film holders. If desired, one section of a series can be 

 mounted on film and a successive section mounted on glass. Sections 

 already mounted on film can be cut out of it and mounted on glass 

 slides in the conventional manner. Section details are clear under low 

 and medium power. For oil-immersion or high-power examination, a 

 cover glass should be mounted on the section with a nondrying immer- 

 sion oil (cedar oil dissolves plastic). Only low-power projection can be 

 used with this film. Pickett and Sommer include the design of a holder 

 to keep the film flat while being scanned under the microscope. 



REQUIRED materials: 



55 mm. film, Cronar, P-40B leader film, E. I. DuPont 



Krylon Plastic Spray, Crystal Clear Spray Coating #1302, Krylon 



Inc., Norristown, Pennsylvania 

 Nikor 35 mm. developing reel, stainless steel 



392 



