410 Solution Preparation (sec. 1) 



ACID ALCOHOL. 



10% ethyl alcohol 100.0 ml. 



hydrochloric acid, concentrated 1.0 ml. 



ALKALINE ALCOHOL. 



70% ethyl alcohol 100.0 ml. 



ammonia, concentrated 1.0 ml. 



(or saturated aqueous sodium or lithium bicarbonate) 



CARBOL-XYLOL. 



phenol (carbolic acid) melted 1 part 



xylene 3 parts 



During use, keep covered to reduce evaporation of xylene. 



For creosote-xylol use beechwood creosote in place of phenol. For 



aniline-xylol use aniline. 



GOLD CHLORIDE STOCK SOLUTION. 



gold chloride 1.0 gm. 



distilled water 100.0 ml. 



lugol's solution. There are various formulas to which this name has 



been applied. 



a. The strongest concentration is: 



iodine crystals 1.0 gm. 



potassium iodide 2.0 gm. 



distilled water 12.0 ml. 



h. Weigert's variation: 



iodine ' 1 .0 gm. 



potassium iodide 2.0 gm. 



distilled water 100.0 ml, 



c. Gram's variation: 



iodine 1-0 gm. 



potassium iodide 2.0 gm. 



distilled water 300.0 ml. 



In all cases, first dissolve the potassium iodide, then the iodine will 

 go into solution readily. 



Physiological Solutions 



Physiological Saline — NaCl in distilled water 

 mammals, O.Q^o (O'gm./lOOO ml. water) 

 birds, 0.757o (7.5 gm./lOOO ml. water) 



