Stock Solutions 411 



salamander, 0.8% (8 gm./lOOO ml. water) 

 frogs, 0.647o (6.4 gm./lOOO ml. water) 



Locke's Solution 



sodium chloride 0.85 gm. 



(for cold-bloods, 0.65 gm.) 



potassium chloride 0.042 gm. 



sodium bicarbonate 0.02 gm. 



calcium chloride 0.024 gm. 



distilled water 100.0 ml. 



Ringer's Solution 



sodium chloride 0.9 gm. 



(for cold-bloods, 0.65 gm.) 



potassium chloride 0.042 gm. 



calcium chloride 0.025 gm, 



distilled water 100.0 ml. 



Best prepared fresh. 



ARTIFICIAL SEA WATER, HALE (1958). 



chlorinity, 19 0/00 

 salinity, 34.33 0/00 



NaCl 23.991 gm. 



KCl 0.742 gm. 



CaCL 1.135 gm. 



(CaCI,-6H20 2.240 gm.) 



MgCL. 5.102 gm. 



(MgCL-GH.O 10.893 gm.) 



NaaSO^ 4.012 gm. 



(Na.SO.-lOHsO 9.1 gm.) 



NaHC03 0.197 gm. 



NaEr 0.085 gm. 



(NaBr-2H.O 0.115 gm.) 



SrCU 0.011 gm. 



(SrClo-GHsO 0.018 gm.) 



H3BO3 0.027 gm. 



Dissolve in distilled water and make up to 1 liter. 



Note: The weights in the right-hand column are for the anhydrous 

 form of the salt; those in parentheses have water of crystallization 

 present, as indicated on the reagent bottle. 



