Adhesive Solutions 41^ 



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Recommended by many for alkaline silver techniques, when sections 

 tend to float off during or after impregnation. Float sections on solu- 

 tion on slide, and place on warm plate. When sections have spread, 

 drain off excess gelatine and blot dry with filter paper. Place in 

 formalin vapor, 40-50 °C overnight. 



Haupt's Gelatine Fixative (1930) 



gelatine 1.0 gm. 



distilled water 100.0 ml. 



Dissolve at 30°C (not above) in water bath or oven. Add: 



phenol (carbolic acid) crystals 2.0 gm. 



glycerol 15.0 ml. 



Stir well, and filter. 



Use 2% formalin when mounting sections. This hardens the gelatine; 

 water is not adeqtiate. Some may find the formalin fumes to be irri- 

 tating to the eyes and nostrils. 



Haupt suggests that if sections tend to loosen, place a small uncovered 

 dish of concentrated formalin in an oven with the slides while drying 

 them. The formalin tends to make the gelatine insoluble and helps 

 to hold the sections in place. 



Weaver's Gelatine Fixative (1955) 



SOLUTION a: 



gelatine 1.0 gm. 



calcium propionate^ 1 .0 gm. 



Roccal (1% benzalkonium chloride) 1.0 ml. 



distilled water 100.0 ml. 



SOLUTION b: 



chrome alum (Cr2Ko(S04)4-24H.O) 1.0 gm. 



distilled water 90.0 ml. 



formalin 10.0 ml. 



Mix in proportions of 1 part of A to 9 parts of B. Flood slide with 

 adhesive mixture, add paraffin ribbons, and allow to stretch as usual. 

 Drain off excess adhesive and blot. Wipe edges close to paraffin. De- 

 posits of adhesive should be removed because it does pick up stain. 

 Good for sections ^vhich are diflicult to affix. 



' Mycoba, manufactured by E. I. Dul'uiii dc Xcmouis and Co., Inc. 



