414 Solution Preparation (sec. \) 



Blood Serum Fixative (prim an 1954) 



fresh human blood serum (only from negative 



Wasserman's) 15.0 ml. 



distilled water 10.0 ml. 



formalin, 5% 6.0 ml. 



Filter through filter paper and use same as albumen fixative. Does 

 not stain and sections do not loosen. 



Buffer Solutions 



Molecular Weights of Buffer Ingredients 



acetic acid, CH3COOH 60.05 



borax, NaoB4O7-10H2O 381.43 



boric acid, B(OH), 61.84 



citric acid (anhydrous), C3H4(OH) (COOH)., 192.12 



citric acid crystals, C3H4 (OH)(COOH)3-H20 210.14 



formic acid, HCOOH 46.03 



hydrochloric acid, HCl 36.465 



maleic acid, HOOCCH-CHOOH 116.07 



potassium acid phosphate, KH2PO4 136.09 



sodium acetate, CHaCOONa 82.04 



sodium acetate, crystals, CH3COONa-3H.O 136.09 



sodium barbital, CsHnOaN^Na 206.18 



sodium citrate, crystals, C3H40H(COONa)3-5m,0 357.18 



sodiuiu citrate, granular, C3H40H(COONa)3-2H,0 294.12 



sodium hydroxide, NaOH 40.005 



sodium phosphate, monobasic, NaH2P04-H20 138.01 



sodiimi phosphate, dibasic, NaoHP04 -71^20 141.98 



sulfuric acid, H,.S04 98.082 



tris (hydroxymethyl) aminomethane CmHuNOs 121.14 



These are a few of the more commonly used chemicals for buffer so- 

 lutions; for others consult a chemical handbook. Nine buffer tables fol- 

 low, and will be adequate for most histological preparations. 



0.2M Acetate Buffer (gomori) (pH 3.8-5.6) 



STOCK solutions: 



acetic acid: 12.0 ml. made up to 1000 ml. with distilled water 

 sodium acetate: 27.0 gm. made up to 1000 ml. with distilled water 

 Add a few crystals of camplior to both solutions. 



