Buffer Solutions 410 



pH citric acid disodium phos])hatc 



milliliters milliliters 



0.2M Tris Buffer (hale) (/;H 7.19-9.1) 



STOCK solutions: 



0.2M tris (hydroxymethyl) (nuiuoruethaue: 24.228 gm. made up to 



1000 ml. with distilled water 

 O.IN HCl (38% assay): 8.04 ml. made up to 1000 ml. with distilled 

 water 

 To 25 ml. 0.2M tris add O.IN HCl as indicated in table below and dilute 

 to 100 ml. 



pH O.IN HCl 



