420 



Solution Preparation (sec. 1) 



Tris Maleate Buffer (gomori) (pH 5.8-8.2) 



STOCK solutions: 



maleic acid 29.0 gm. 



tris (hydroxymethyl) aminomethane . . . . 30.3 gm. 



distilled water 500.0 ml. 



Add 2 gm. charcoal, shake, let stand 10 minutes and filter. To 40 ml. 

 of stock solution add N NaOH (■/%) as indicated below and dilute to 

 100 ml. 



pYL 



sodium hydroxide 



Stain Solubilities 



acid alizarine blue 

 acid fuchsin 

 acridine orange 

 acridine yellow 

 acriflavine 

 alcian blue 

 alizarine red S 

 aniline blue ws 

 auramin 



in water 



per cent 



in absolute ethyl alcohol 

 per cent 



{from {from {from {from 



Conn, 1953) Gurr, 1960) Conn, 1953) Gurr, 1960) 



26°C 



7.69 



0.74 



15°C 



1.0 



45.0 



5.0 



0.5 



15.0 



9.5 



6.5 



50.0 



1.0 



26°C 



0.15 



4.49 



15°C 



0.75 



3.0 



0.75 



0.75 



1.0 



6.0 



0.15 



0.0 



4.0 



