428 General Laboratory Aids (sec. 2) 



chlorate hydrate . . 300.0 gm 



potassium sulfate 6.0 gm. 



potassium nitrate 1 14.0 gm. 



sodium sulfate 54.0 gm. 



sodium chloride 66.0 gm. 



sodium bicarbonate 60.0 gm. 



formalin 300.0 ml. 



distilled water 10,000.0 ml. 



Because of contained air, lungs will float on the fixative. Submerge 



them under cotton soaked with fixative. 



Drain off excess Kaiserling and place specimens in four times their 



volume of 80% alcohol: 18-24 hours. 



Deep freeze at — 29°C±, sealed in air-tight polyethylene bags not 



much larger than the specimen. 



The specimens will keep indefinitely this way, retain their color, and 

 become pliable after thawing. After use, they can be returned to deep 

 freeze with little deterioration. 



Removing Laboratory Stains from Hands 

 and Glassware 



STAIN TREATMENT 



basic juchsin — difficult to remove; try strong acetic acid in 95% alcohol, 

 or dilute HCl. 



carmine — strong ammonia water or weak HCl; if stain resists, use them 

 alternately. 



chromic acid — dilute sulfurous acid in water, or concentrated sodiimi 

 thiosulfate and a few drops of sulfuric acid added. 



jast green and other similar acid stains — ammonia water. 



hematoxylin — weak acid or lemon juice. 



hemoglobin — fresh stains: lukewarm to cool water (never hot); older 

 stains: soften with borax solution, dilute ammonia or tincture of 

 green soap, finally treat with 2% aqueous oxalic acid. 



iodine — sodiimi thiosulfate. 



iron alum stains on glassumre — (1) strong NaOH (sticks) in water, fol- 

 lowed by (2) strong HCl. 



methylene blue — acid alcohol or tincture of green soap. 



most dyes — tincture of green soap. 



osmic acid on glassware — 3% HoOo [Carr and Bacsich, 1958). 



