460 



Index 



Champy-Kull modification of Altmann- 



Benda for mitochondria, 264 

 Chelating agents for decalcification, 27 

 Chick embryos, fixing and staining, 378 

 Chitin staining, 148 

 Chloral hydrate for anesthetizing, 372 

 Chloral hydrate silver method: for nerve 



fibers and endings, 205; for pericellular 



end-bulbs, 206 

 Chloretone for anesthetizing, 372 

 Chlorinity, definition, 409 

 Chloroform: for anesthetizing, 372; for 



clearing, 34; for double embedding, 84 

 Chromaffin material, 250 

 Chromatization, after fixation, 23 

 Chromic acid, see Chromium trioxide 

 Chromium hematoxylin phloxine method 



for pancreas, 290 

 Chromium trioxide: in fixatives, 6, 400; with 



Schiff's reagent, 301, 321 

 Chromogen, 11 1 

 Chromophorcs, 1 1 1 



Chromosomes: permanent mounts, 369; res- 

 toration of slides, 370; squash and smear 



technics, 367; temporary mounts, 368 

 Chromotrope 2R, in trichrome method, 157, 



158, 159, 389 

 Chrysoidin staining of mast cells, 277 

 Churg and Prado trichrome method, 158 

 Ciliates, anesthetizing, 372 

 C.I. number, 113 



Citric acid-citrate decalcification, 25 

 Cleaning slides, 423 

 Cleaning solution for glassware, 412 

 Clearing for paraffin method, 33 

 Clinical microtome, 43, 64 

 CMC-10 mounting medium, 121 

 Cocaine for anesthetizing, 372 

 Cochineal, source, 105 

 Coelenterates: anesthetizing, 372; fixation, 



373 

 Cold chamber (cryostat), 337 

 Collagen combined with reticulum and 



elastin staining, 186 

 Collagenic fibers (collagen): definition, 145; 



staining, 1 18, 150, 152, 153, 155, 159, 160, 



162, 164, 165, 167, 187,231 

 Collodion, see Nitrocellulose 

 Colloidal iron for acid mucojjolysaccharides, 



270 

 Colophonium, 225 

 Color index nimiber, 113 

 Colorless tissue, special treatment for em- 

 bedding. 32 

 Commission Certified, 113 

 Congo red: counterstain, 130; amyloid stain, 



274 

 Connective tissue methods, 145 

 Controlled illumination, 90 



Coon's fluorescent technic for antigen-anti- 

 bodies, 331 

 Corals: anesthetizing, 371; fixation, 373 

 Corrosive sul)limate, see Mercuric chloride 

 Counterstains: definition, 114; listing of, 129 

 Cover glasses, microscopic: cleaning, 57; de- 

 scription, 56; mounting, 115, 122; ringing, 

 123 

 Creo.sote-xylol, see Carbol-xylol 

 Cresyl violet for Nissl substance, 195 

 Critical illumination, Kohler method, 91 

 Cryostat cold chamber, 337 

 Crystallization of paraffin, 37 

 Crystal violet staining, 307-310; for amyloid, 

 278; capsule staining, 317; trichrome 

 method, 158 

 Cyanol reaction for hemoglobin, 239 



Dafano fixative, 17 



Dafano method for Golgi, 260 



Dark field microscopy, 96 



Darrow red nuclear stain, 139 



Decalcification: acid reagents, 25; chelating 

 agents, 27; combined with other processes, 

 28; electrolysis method, 26; ion-exchange 

 method, 26 



Decalcification-dehydration, 29 



Deformalization, after fixation, 24 



Degenerating axons: Nauta and Gygax 

 method, 208; Marchi method, 210 



Dehydrants, 31 



Dehydration and clearing combined for 

 paraffin method: Dioxane method, 39; 

 Tetrah)drofuran method, 40 



Dehydration combined with fixation, 29 



Dehydration: for embedding, nitrocellulose 

 method, 71; paraffin method, 30; during 

 staining, 114, 131 



Delafield's hematoxylin: formula, 124; pro- 

 gressive method, 130; regressive method, 

 133 



Del Rio Hortega methods of silver impreg- 

 nation, 176; for neurological elements, 

 194; for reticulum, 180 



Desoxyribonucleic acid, polysaccharides 

 and protein, triple stain for, 302 



Dialyzed iron, 271 



Diamond knives for electron microscopy, 403 



Diaphane mounting medium, 122 



Diaphragm: iris, 90; annular ring, 97 



Diazo-safranin method for enterochromaffin 

 cells, 250 



Dieterle's method for spirochetes, 317 



Difficulties in sectioning: freezing method, 

 64; nitrocellulose method, 76; paraffin 

 method, 54 



Diluting alcohol for dehydration and hydra- 

 tion, 31 



Di-nitro.'joresorcinol method for iron, 236 



