Index 



401 



Dioxane: as dch\(lr:int, 31; flnuhle cinl)cd- 

 (liiig, 85; paraffin nictluui, ?>\): toxicity, 39 



DNA, desoxyritiose nucleic acid, methods 

 for, 294, 360 



Dopa oxidase reaction, 351 



Double embedding methods, 83 



Dryers, hot air slide, 60 



Dry ice holder, 62 



Drying resin mounts, 1 18 



Dyes, see Stains 



Eartliwonns: anesthetizing, 372; fixation, 

 375 



Echiiioderniata, fixing, 377 



Egg all)iimen: formula, 412; use, 58 



Ehrlich's hematoxylin, 124 



Elastin combined vvitli reticulum and colla- 

 gen staining, 186 



Elastic fibers (elastin): definition, 145; stain- 

 ing of, 152, 160, 162, 164, 165, 166, 167. 

 168, 169, 187 



Electrolysis mctiiod for decalcification, 26 



Electrol) tic dissociation of dyes, 1 1 1 



Election microscopy: description, 103, 397; 

 fixatives for. 399; knives for, 403; section- 

 ing, 403 



Elementary and inclusion bodies, 306 



Embedding: double methods. 83; electron 

 microscopy, 401; for enzymes, 339; gela- 

 tine. 66; nitrocellulose, 72; paraffin, 36; 

 polvester resin, 85; water soluble waxes, 

 80 



Embryos: alizarine red S method. 172: fixing 

 and staining. 382 



Endogenous pigments, 232 



Enterochromaffin cells, 250; a/o-coupling 

 method, 252; diazo-safranin method, 250; 

 fcrric-ferricyanide method, 251; silver 

 method, 248 



Enzymes, acetone fixation and embedding 

 of, 339 



Eosin-azure stain (Maximow's), 227 



Eosin; counterstaining, 129; cosin-orangc, 

 129; Putt's, 129; with hematoxylin, 131 



Eosinophils (leukocytes), 220, 229 



Epoxy embedding, electron microscopy, 401 



Erythrocytes, 218! 220, 228 



Erythrosin B counterstain, 130 



Esterase and Lipase, 347 



Ethanol, see Ethyl alcohol 



Ether for anesthetizing invertebrates, 372 



Ethyl alcohol: as dehydrant, 31; fixatives, 6 



Euparol, 1 16 



Exfoliative cytology, 356; fluorescent 

 method, 359; Papanicolaou method, 357 



Exogenous pigments, 232 



Faded slides, restaining, 424 



lanz knife sharpener (A. H. Thomas), 46 



Farrant's mounting medium, 1 19 



Fast green: counterstain, 130; in Feulgen 

 stain, 291; Masson stain, 153; for mito- 

 chondria, 262; in Quad slain, 161; for sec 

 chromatin, 366 



Fat staining: discussion, 253; methods, 254 



Ferric-ferricyanide: for enterochromaffin, 

 251 ; for melanin, 242 



Ferrous iron, Turnbull blue method, 235 



Feulgen technics: discussion, 292; methods, 

 293. 301 



Fibrin: definition, 116; staining, 156, 159, 

 164 



Field's method and stain for blood smears, 

 221 



Film mounts: of sections, 392; smears and 

 tissue cultures, 393 



Films, teaching, 429 



Fite formaldehyde method for bacteria, 314 



Fixation, 3; with decalcification, 28; for 

 enzymes, 339; perfusion, 22; post-fixation, 

 23; precautions and preparations, II; 

 washing after, 12 



Fixatives (adhesives). 412 



Fixing solutions: chemicals used, 5; for elec- 

 tron microscopy, 399; for enzymes, 339; 

 routine solutions, 13; special solutions, 16 



FIcmming's fixatives, 17 



Flukes: anesthetizing, 372; fixation, 373; 

 staining, 382 



Fluorescent methods: for amyloid, 275; for 

 antigen-antibodies, 331; apparatus, 102; 

 for DNA, 295; for exfoliative cytology, 

 359; for fat, 257; for fungi, 323; mounting 

 media, 122; for mucin, 273; for neurologi- 

 cal tissue, 202; for tubercle bacilli, 316 



Fluorochromes, 101; uses for, 103 



Foreign stains, sources, 113 



Formaldehyde: pigment, 7, 241; use in fixa- 

 tives, 7, 14 



Formalin, see Formaldehyde 



Formic acid decalcification, 25; N solutions, 

 408 



Formol-alcohol fixative, 18 



Formulas, fixatixes, 13 



Freeze-drying for eiribedding, 336 



Freeze-substitution, see Ice-sohent method 



Freezing method, 62; surgical Iiiopsies, 63; 

 rapid staining, 67 



Freon, use of, 62 



Fungi staining. 300, 320 



Gallocyanin: formula, 128; for N'issI sui)- 

 stance, 197; for sex chromatin, 3()5; stain- 

 ing procedure, 138 



(;arnet GBC salt: to purify, 346; use, 252, 

 , 345 



Ciastropods, anesthetizing, 372 



C.clatine embedding, freezing method, 66 



