464 



Index 



Metachromasia: acid mucopolysaccharides, 

 280; amyloid, 278; discussion, 278; mast 

 cells, 281; mucin, 279 



Metachroming, 110 



Metanil yellow counterstain, 322 



Methacrylate embedding, election micros- 

 copy, 402 



Methenamine silver nitrate for: entero- 

 chromaffin cells, 248; fungi, 322; uric acid, 

 248 



Methyl benzoate, use in double embedding, 

 83 



Methylene blue: with acid fast stains, 312; 

 Loeffler's, 317; for Negri bodies, 328; with 

 phloxine, 229; for Rickettsia, 326 



Methyl green: counterstain, 263; for nu- 

 cleic acids, 297 



Methyl salicylate for clearing, 34 



Microfilaria, fixation and staining, 375 



Microglia: silver impregnation, 194; stain- 

 ing, 191 



Micrometers, ocular and stage, 97 



Microorganisms, discussion of, 304 



Microscope: compound, 88; dark field, 96; 

 electron, 103; fluorescent, 101; measuring 

 devices, 94; operation, 91; phase and in- 

 terference, 97; polarizing, 98; ultraviolet, 

 100; X-ray, 99 



Microscope lamps, 90 



Microscopic cover glasses: cleaning, 57; de- 

 scription, 56; double cover glass mount- 

 ing, 122; single glass mounting, 115 



Microscopic slides: cleaning, 57; descrip- 

 tion, 56; labelling, 423; see also Mounting 



Microscopy: brightfield, 88; dark field, 96; 

 electron, 103, 403; fluorescent, 101; inter- 

 ference, 97; phase, 97; polarizing, 98; 

 ultraviolet, 100; X-ray, 99 



Microtome knives, 44 



Microtomes: kinds, 43, 62; use of in freezing 

 method, 62; nitrocellulose method, 74; 

 paraffin method, 52. 



Millon reaction for protein, 246 



Mitochondria: discussion, 259; methods 

 for, 262 



Molds for paraffin embedding, 36 



Molecular solutions, definition, 407 



Molecular weights of buffer ingredients, 414 



Molluscs: anesthetizing, 372; fixing, 376 



Monocytes, staining, 220 



Mordants and mordanting: discussion, 107; 

 destaining with, 108, use, 107 



Motor end plates. Cole method, 211 



Mountants, See Mounting media 



Mounting a cover glass, 115; aqueous 

 mounts, 122; difficulties, 116; two cover 

 glass method, 123 



Mounting media, 116; quick drying of, 118 



Mounting media formulas: Apathy's gum 

 syrup, 119; Berlese, 120; Farrant's, 119; 

 fluorescent, 122; Gray and Wess', 120; 

 histochemical, 355; Kaiser's ghcerol jelly, 

 119; Karo medium, 121; lactophenol, 121; 

 polyvinyl alcohol (PVA), 120; Yetwin's 

 for nematodes and ova, 121 

 Mounting sections: freezing method, 65; 

 nitrocellulose method, 77; paraffin 

 method, 51 

 Movat's pentachrome stain, 162 

 Mucicarmine (Mayer's) for mucin, 273 

 Mucin: metachromatic methods, 279; re- 

 moval of, 300; staining, 273 

 Mucopolysaccharide (acid): staining, 269; 



metachromatic methods, 280 

 Mucus staining, 148, 152, 153, 155, 156, 360 

 Muscle staining, 148, 150, 153, 155, 156, 159, 



160, 162, 280 

 Mussels, preparation for fixing, 376 

 Myelin staining, 212, 241; Luxol fast blue- 

 Holmes silver, 214; Luxol fast blue-PAS, 

 212; Mahon method, 217; Ora's method, 

 215 



Naphthalene yellow for fat, gross specimens, 

 257 



Naphthol yellow S, 302 



Narcotizing invertebrates, 371 



Nassar and Shanklin's silver impregnation 

 for neuroglia, 192 



Natural dyes, 105 



Nature of staining action. 111 



Nauta and Gygax method: degenerating 

 axons, 208; pericellular end bulbs, 207 



Xavashin's fixative, 20 



Negri body staining, 328 



Nemathelminthes, fixation, 374 



Nematodes: fixation, 374; mounting me- 

 dium for, 375 



Nemertinea, fixation, 374 



Nerve cells, fibers and endings: Bielschow- 

 sky method, 201; Bodian method, 203; 

 chloral hydrate silver method, 205; 

 Golgi's rapid method, 198; Ramon y 

 Cajal's pyridine silver method, 200 



Neurofibrils, Ram6n y Cajal's method, 197 



Neurological elements, staining: astrocytes, 

 193; degenerating axons, 208; fluorescent 

 method, 202; motor end plates, 211; 

 myelin, 212; nerve cells and fibers, 198; 

 fibers and endings, 203; neurofibrils, 197; 

 neuroglia, 192; Nissl substance, 195; 

 nomenclature, 189; oligodendroglia and 

 microglia, 194; preparation for staining, 

 190; silver methods, 192; victoria blue 

 staining, 191 



Neutral red staining of mast cells, 276 



Neutral stains, 110 



