Index 



46 



),) 



Neiitrnphils ('lciikoc\ tes), staininjz;. 220 

 New fuchsin: with orcinol. clastin stain, 



167; carbol -new fuchsin, 313, SI,") 

 Nile blue A: for fats and fattv acitls, 258; 



for melanin and lipofuscin, 241 

 Ninhydrin reaction for protein, 247 

 Nissl substance methods: cresyl violet, 195; 



gallocyanin, 197; thionin, 196 

 Nitric acid: decalcification, 25; N solutions, 



408 

 Nitrocellulose, for loose paraffin sections, 60 

 Nitrocellulose method, 70; difficulties in 



sectioning, 76; double embedding, 83; 



hot celloidin method, 75; serial sections, 



78; staining and mounting, 76 

 Nitrogen, liquid, use for freeze-drying, 336 

 Normal solutions: definition, 407; dilutions 



for acids, 408 

 Nuclear staining, red, 139 

 Nucleic acids, 293 

 Nudibranchs, anesthetizing, 371 



Obelia, staining, 377 



Objectives of microscope, 89 



Occult iion, see Masked iron 



Ocular micrometer, 95 



Ocular, pointer for, 94 



Oil immersion oljjective, use and care, 93 



Oil of Wintergreen, see Methyl salicylate 



Oil red O for fat, 254 



Oils for clearing, 34 



Oligodendroglia siher impregnation, 194 



Omentimi, see SubciUaneous tissue 



Orange G: counterstain, 130, 230, 285, 287; 



in Mallory's stain, 147; in Quad stain, 161 

 Ora's method for myelin, 215 

 Orcein: stain for elastin, 161, 169; source 



of, 106 

 Orcinol-New fuchsin elastin stain, 167 

 Ordway-Machiavello method for rickettsia 



and inclusion bodies, 326 

 Orthochromatic action, 111 

 Orth's fixative, 15; Lillie's variation, 15 

 Osmic acid staining, see Osmium tetroxide 

 Osmium tetroxide in fixatives, 8, 399 

 Osmimn tetroxide staining: of fat, 256; of 



Golgi apparatus, 259; of mitochondria, 



266 " 

 Ova, mounting medium for, 375 

 Oxidation of hematoxylin, 105 



Pancreas staining, 290 



Papamiltiades hematoxylin, formula, 125 



Papanicolaou method for exfoliative cy- 



tolog)', 357 

 Paper mold for paraffin embedding, 37 

 Pappenhcim stain for rickettsia, 324 

 Paraffin blocks: hardening, 36; sectioning, 



52; storing, 38; trimming-mounting, 51 



Paraffin: discussion, 35; rrvstalli/ation. 37; 

 infiltration and embedding, 35 



Paraffin method: clearing, 34; dehydration. 

 30; Dioxane with, 39; double embedding, 

 83; infiltrating and embedding, 35; 

 mounting slides, 57; sectioning, 52; Tet- 

 raliydrofuran with, 40; tiining schedule, 

 41; tissue processors, 42; trimming and 

 blocking, 51 



Paraffin sections: detaching, 60; difficulties 

 in sectioning, 54, 58; mounting, 51; static 

 elimination, 54; storing, 54 



Paramecium, fixing and staining. 384 



Parasites: description, 386; intestinal pro- 

 tozoa, smear technics, 386; section technic, 

 390 



Parenchyma, definition, 145 



Parfocal, 92 



Parloidin, see Nitrocellulose 



PAS, see Periodic acid Schiff 



Pencils, glass marking, 57 



Penfield's modification of del Rio Hortega's 

 siher impregnation, 194 



Pentachrome stain, Movat's, 162 



Peracetic acid, 243 



Percentage solutions, definition, 408 



Perenyi's fixative, 20 



Performic acid, 243 



Perfusion, fixation by, 22 



Pericellular end-bulbs (boutons): chloral 

 hydrate-silver method, 206; Nauta and 

 Gygax method, 207 



Periodic acid Schiff: discussion, 292; for 

 fiuigi, 300; for parasites, 386; methods, 

 298 



Periodic acid solutions, 296, 299, 300, 302 



Perl's reaction, see Berlin blue 



Permount, 116 



Phase and interference microscopy, 97 



Phenol new fuchsin, formula and use, 313, 

 315 



Phloxine B counterstain, 130, 291 



Phloxine-Methylene blue stain, 229 



Phosphate buffer: Gomori's, 417; Sorenson's, 

 417 



Phosphine 3 R for fat staining. 257 



Phospholipids, Sudan black method, 258 



Phosphomolybdic acid: in Mallory type 

 stain, 147; in Masson stain, 152, 154; in 

 Quad stain, 161 



I'hosphotungstic acid: in Mallory type stain, 

 147, 150; in Masson stain, 152; in Penta- 

 chrome stain, 163; in Quad stain, 161 



Phosphotungstic acid hematox)lin: for- 

 mula, 125; staining procedure, 136 



Photoxylin, see Nitrocellulose 



Physiological solutions: definitions, 409; 

 formulas, 410 



I'iccolvtc, 116 



