Index 



467 



Saccharides, description, 266 



Saffron: in pcntachrome stain, 163; source, 

 106 



Safranin counterstain, 233 



Salinity, definition, 409 



Sanfelice fixati\e, 21 



Scarba red nuclear stain, 140 



Schaudinn's fixati\e, 21; PVA modification, 

 389 



Schedule for timing paraffin method, 41 



Schleifstein melhod, 328 



Schiff's reagent: formulas, 293-295; Azure 

 A reagent, 302; Huorescent, 296 



Schmorl technic. 251 



Scott's solution: formula, 412; use, 131 



Sea anemones: anesthetizing, 371; fixation, 

 373 



Sea water, artificial, 41 1 



Section mounting: freezing method, 65; 

 nitrocellulose method, 77; paraffm 

 method, 51 



Sectioning: electron microscopy, 403; freez- 

 ing method, 64; nitrocellulose, 74; paraf- 

 fin, 52 



Sectioning difficulties: freezing method, 64; 

 nitrocellulose method, 76; paraffin 

 method, 54 



Sections detaching, 60 



Serial sections: nitrocellulose, 78; paraffin, 

 60 



Serum, blood, adhesive, 414 



Sex chromatin, methods for, 362 



Sharpening microtome k.ni\es, 45 



Silver impregnation: discussion, 175; loose 

 sections, 178; neurological methods, 192; 

 reticulimi metliods, 179 



Sinha's fixative, 21 



Sledge microtome, 44 



Slide dryers, hot air, 60 



Slide mounting: freezing method, 65; nitro- 

 cellulose method, 77; paraffin method, 51 



Slides, microscopic: cleaning, 57; descrip- 

 tion, 56; labelling, 423; see also Mount- 

 ing 



Slide warmer, 58 



Sliding microtome, 43, 74 



Smith's fixative, 21 



Snails, killing and fixing, 372, 376 



Sodium thiosidfatc, 412 



Solubilities of stains, 420 



Solution preparation, 407, 409 



Sources of foreign stains, 113 



Spirochete staining, 317 



Sponges, fixation, 372 



Stage micrometer, 95 



Stain commission, 112 



Staining action: acido}>liilic, 110; baso- 

 philic, 110; double, 109; general consider- 

 ations, 104; metachroming, 110; nature 



of. 111; orthochroming, 111; polvdirom- 

 ing, 110; progressive, 108, 130; regres- 

 sive, 108, 133: triple, 110 



Staining procedures: freezing method, 67; 

 general principles. 114; nitrocellulose, 76; 

 paraffin method. 130 



Stain removal: from hands and glassware, 

 428; from slides, 424 



Stains: C.f. number, 113; discussion, 104; 

 natural, 105; neutral, 110; nomenclatine, 

 111; Romanowsky, 110; solubilities, 420; 

 standardization, 112; synthetic, 109 



Standard buffer, Mcllvaine's, 418 



Standardization of stains, 112 



Static elimination, paraffin sectioning. 54 



Sterling's gentian violet, formula and use, 

 307,311 



Stieve's fixative, 15 



Stock solutions, 409 



Storing gross specimens, 426 



StiDina, definition, 145 



Strops, care and use, 47 



Subcutaneous tissue, preparation and stain- 

 ing, 1()9 



Succinic dehydrogenase, 350 



Sudan staining: for fat, 255; for phospholip- 

 ids, 258 



Sulfuric acid N solutions, 408 



Surgical biopsies freezing method, 63 



Susa's fixative, 15 



Synthetic dyes, 109 



Synthetic resins, 116; quick drying of, 118 



Tapeworms: anesthetizing, 372; fixation, 

 373; staining, 377, 382, 384 



Teaching films, 429 



Tellyesniczky fixative, see Formol-akoliol 



Testing hematox\lin solutions, 128 



Tetrahydrofuran method for parallin em- 

 bedding, 40 



Thermoplastic mounts, 394 



Thick blood smears, preparation and stain- 

 ing, 221 



Thin blood smears, preparation and stain- 

 ing, 219 



Thioflavine T, for ani) loid, 275 



Thionin: acid mucopolysacciiarides, 280; 

 bone, 170; freezing mctliod, 68; mast 

 cells, 281; metachroming, IK); nuiciu, 

 280; Nissl substance, 197; sex chromatin, 

 364 



Thymonucleic acid, see Nucleic acids 



Timing schedule, paraffin method, 41 



Tissue processors, automatic, 42 



Toluene clearing: for paraffin melhod, 33; 

 after staining, 1 15 



Toluidine blue staining. 238; freezing 

 method, 68; mast cells, 282; mucin, 279 



Touch preparations, 29 



