CONTENTS 



Page 



Urea Silver Nitrate, for nerve fibres and nerve endings 217 



Verhoeff's Stain for elastic fibres, nuclei and collagen . . 218 



Water Blue - Orcein - Safranin for demonstrating epith- 

 elial fibres . . . . . . . . . . . . . . 220 



Weigert - French Elastin Stain (Moore's modification) 



for elastic tissues . . . . . . . . . . . . 221 



Weigert - Pal Technique for myelin in brain and spinal 



cord and for peripheral nerves and ganglia . . . . 223 



Wool Green - Haematoxylin - Ponceau S. for connec- 

 tive tissue and muscle . . . . . . . . . . 224 



Wright's Stain for general differentiation of blood cor- 

 puscles ; for malarial parasites ; trypanosomes, etc. . . 225 



SECTION 3— BOTANICAL METHODS 

 Normal and Infected Tissues 



{a) GENERAL TREATMENT OF TISSUES . . . . 229 



(h) MISCELLANEOUS MICROCHEMICAL TESTS 230 

 Aldehydes — Aleurone — Amygdalin — Amylodextrine 

 — Anthocyanin — Arbutin — Asparagine — Calcium — 

 Calcium Oxalate — Callose — Carotin — Cellulose — 

 Chitin — Chlorides — Chlorophyll — Formic Acid — 

 Glutathione — Inulin — Iodine — Iron — Lecithin — 

 Nitrates — Pectic Substances — Phosphates — Phyto- 

 sterol — Potassium — Proteins — Saponin — Sodium — 

 Sulphates — Tyrosin. 



{c) STAINING TECHNIQUES 247 



Acid Fuchsin - Aurantia for differentiating between bac- 

 teria and mitochondria in infected tissues . . . . 247 



Acid Rubin - Aurantia - Toluidine Blue (KuU's Stain), 



for starch grains and mitochondria . . . . . . 248 



Aniline Hydrochloride, a rapid method for demonstrating 



lignified tissues . . . . . . . . . . . . 249 



Basic Fuchsin, Ammoniacal for lignified walls and cutin . . 249 

 Borax Carmine (Grenacher) for bulk staining and for small 



whole mounts . . . . . . . . . . . . 250 



xviii 



