CONTENTS 



Page 



3. Intravital Staining . . 



4. Muscle 



5. Nerve Tissues, Differential Staining of 



6. Soil Bacteria, Staining and Counting ; Differentia- 



tion OF Living from Dead Cells 



7. Trypanosomes, Living, in Blood 



8. Trypanosomes, in Dried Blood Films 



9. Tubercle Bacilli 



10. Virus Staining . . 



SECTION 6— HISTOCHEMICAL METHODS 



319 

 320 



321 



322 



323 



324 



325 

 326 



Micro- Incineration for the detection and location of 



mineral salts .. .. .. .. .. ..331 



3 -Hydroxy - 2-Naphthoic Acid - Tetrazotized o-Ani- 



sidine, for demonstrating sites of carbonyl activity . . 333 



Metanil Yellow - Iron Haematoxylin for radioauto- 



graphs . . . . . . . . . . . . . . 334 



Methyl Green - Pyronin - Ribonuclease (Brachet), for 

 detecting ribonucleic acid and desoxyribonucleic acid 

 in the same cell . . . . . . . . . . . . 336 



Nile Blue Sulphate - Safranin, for phospholipids . . 340 



Phosphomolybdic Acid - Eosin, for localization of choline 



containing lipids . . 341 



Sudan Black 342 



SECTION 7— SMEAR PREPARATIONS 



Albert's Stain (Layboum's modification) for diphtheria 



bacilli . . . . . . . . . . . . . . 345 



Alcian Blue, for bacterial capsules and polysaccharides . . 346 



Aniline Blue - Eosin B, for spermatozoa 347 



Aniline Gentian Violet, a rapid stain for Treponema 



pallida . . . . . . . . . . . . . . 348 



AzuR L for the detection and staining of epidermophytic 



infection . . . . . . . . . . . . . . 349 



xxii 



