MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Formula: 



Absolute alcohol . . . . . . 60 ml. 



Chloroform . . . . . . . . 30 ml. 



Glacial acetic acid . . . . . . 10 ml. 



Technique: 



Animal Tissues. Fix for one and a half to two hours; then 

 transfer to two changes of absolute alcohol before clearing and 

 embedding. 



Plant Tissues. Fix root tips for a quarter-of-an-hour ; anthers, 

 for one hour. 



Remarks: 

 A rapid fixative with great penetrating power. 



Carnoy - LeBrun Fluid 



Recommended for : 



Insects and ticks, and as a rapid and penetrating fixative for 

 plant tissues. 



Formula: 



Absolute alcohol . . . . . . 30 ml. 



Glacial acetic acid . . . . • • 30 ml. 



Chloroform . . . . . . . . 30 ml. 



Mercuric chloride . . to saturation (about 10 gm.) 



Technique: 

 Fix from half to one minute ; then wash in 95% alcohol. 



Remarks: 



Not reconmiended for routine fixation of plant tissues. 



Champy's Fluid 



Recommended for : 



Plant and animal tissues in general; mitochondria and other 

 cytological detail. 



Formula: 



Potass, dichromate 3% aqueous . . 35 ml. 

 Chromic acid 1% aqueous . . . . 35 ml. 



Osmic acid 2% . . . . . . 20 ml. 



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