MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



of Doetschman's fluid on a slide. For permanent mounts the 

 preparations should be placed in the oven at 37-50° C. from six 

 to twenty-four hours. Ring the slides with a waterproof cement. 



Reference: MicroscopisV s Vade-Mecum nth ed., p. 207. 



GLYCERINE 



Used as a mounting medium for frozen sections. Only the 

 purest neutral glycerine should be used. Permanent preparation 

 may be made by painting the edges of the coverslips with melted 

 glycerine jelly and coating the jelly, when it has set, with gold 

 size or asphalt varnish. 



GLYCERINE JELLY 



For fat preparations, frozen sections, gelatine sections, etc. This 

 mountant, which should be neutral in reaction, sets sufficiently 

 hard to permit direct varnishing of the edges of the cover«lips, of 

 mounted preparations, without prior luting. Preparations mounted 

 in glycerine jelly will last for many years without deterioration. 



GLYCHROGEL MOUNTANT 



For Marchi-stained sections, gelatine sections, teased pre- 

 parations, nematodes etc. 



A. Chrome alum . . . . . . 0-2 gm. 



Distilled water . . . . • . 30 ml. 



Dissolve by warming. 



B. Gelatine granules . . . . • • 3 g^i- 

 Distilled water . . . . • • 50 ml. 

 Glycerine . . . . . . . . 20 ml. 



Warm the water to 45° C. then shake in the gelatine a little 

 at a time until dissolved and add the glycerine. 



Add solution A to solution B; shake thoroughly; then filter and 

 add about o-i gm. camphor as a preservative. 



Keep in a well-closed bottle to prevent evaporation. 



Reference: Wotton, R. M. and Zwemer, R. L. (1935), Stain Tech., 10, no. i, 

 21-2. 



40 



