SECTION TWO 



Note: The solution turns purple in 24 

 hours, and is ripe and ready for use after being 

 kept at 20° C. for three days or in two days if 

 kept at about 37° C. The stain deteriorates 

 after four or five days. 



E. Ehrlich Haematoxylin 



F. Ethyl alcohol 70% .. .. .. 99-5 ml. 



Hydrochloric acid, cone. . . . . 0-5 ml. 



G. Lithium carbonate, saturated aqueous 



H. Orange G 2% aqueous . . . . 50 ml. 



Light Green SF 1% aqueous . . 20 ml. 



Distilled water . . . . . . 30 ml. 



Chromotrope 2R . . . . . . 0-5 gm. 



Phosphotungstic acid . . . . 0-5 gm. 



Glacial acetic acid . . . . . . i ml. 



Dissolve the phosphotungstic acid in the 

 distilled water, then add and dissolve the 

 chromotrope 2R followed by the acetic acid. 

 Orange G and Light green solutions. Shake 

 thoroughly. 



Note: This solution keeps indefinitely. 



I. Acetic Acid 0*2% 



Technique: 



1. Fix in solution A for 24 hours. 



2. Wash in running tap water for six to eight hours. 



3. Dehydrate, clear and embed in paraffin wax in the usual 

 manner. 



4. Cut sections, in the horizontal plane, 3 to 4jLt in thickness. 



5. Remove paraffin wax from sections with xylol. 



6. Pass through the usual descending grades of alcohol to dis- 

 tilled water. 



7. Immerse in Lugol's iodine for thirty minutes. 



8. Transfer to the sodium thiosulphate solution until the 

 sections have regained their natural colour (about two minutes). 



9. Rinse thoroughly in distilled water. 



51 



