MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



AMMONIACAL SILVER CARBONATE 



For vascular reticulum, tumour cells, connective tissues 

 around tumour, in abnormal brain tissue 



Solutions required: 



A. Pyridin, pure . . . . . . 2 volumes 



Glycerin, pure . . . . . . i volume 



B. Ammoniacal Silver Carbonate. 



Ammonia solution is added drop by drop to 10 ml. 

 silver nitrate io-2% until the precipitate formed is 

 almost redissolved, leaving a slightly opalescent 

 solution to which is then added 10 ml. sodium car- 

 bonate 3-1% solution and sufficient distilled water to 

 make the volume up to 100 ml. 



C. Reducing solution: 



Sodium carbonate anhydrous . . i gm. 

 Formalin . . . . . . . . i ml. 



Distilled water . . . . . . 103 ml. 



D. Brown gold chloride 0-2% aqueous. 



E. Intensifying solution: 



Oxalic acid 2% aqueous . . 100 ml. 



Formalin . . . . . . . . i ml. 



F. Sodium hyposulphite 10% aqueous. 



N.B. — All the above solutions must be kept in 

 dark bottles. 



Technique: 



The material is fixed in 10% formalin or in Bouin and embedded 

 in paraffin wax. 



1. Bring sections down to distilled water and immerse in Solu- 

 tion A for twenty-four hours. 



2. Wash with 95% alcohol, then with distilled water. 



3. Immerse in Solution B for two and a half hours at 40° C. 



4. Wash with distilled water; then reduce in Solution C for 

 five minutes, afterwards washing in tap water. 



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