SECTION TWO 



5. Tone for five minutes in Solution D at 30° C; then wash in 

 tap water. 



6. Intensify by immersing in Solution E for five minutes ; then 

 rinse in tap water. 



N.B. — The above stages must be carried out in the darkroom. 



7. Fix in Solution F. {Note: Fixation should be completed in 

 fifteen to twenty minutes.) 



8. Wash in tap water; dehydrate; clear and mount. 



Results: 



Tumour cells: reddish to greyish violet. Vascular reticulum: 

 black. 



Important. — ^The tissues must not be allowed to come 

 into contact with mercuric chloride, as even a trace will 

 ruin the preparation. 



ANILINE BLUE - ACID FUCHSIN 

 For elementary bodies in animal sections 



Technique: 



I. Pieces of tissue are fixed for twenty-four hours in Solution A; 

 washed, dehydrated, cleared and embedded in paraffin wax as 

 usual. 



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