MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



2. Sections, not thicker than 5^, are fixed to slides, de-waxed and 

 taken through descending grades of alcohol down to water as 

 usual. 



3. Stain for one half to one hour in the aniline blue (Solution B) 

 in a stoppered staining jar. 



4. Rinse well with distilled water. 



5. Drain and blot carefully to remove excess water. 



6. Rinse in absolute alcohol. 



7. Stain for twenty minutes in the acid fuchsin (Solution C). 



8. Pour off; drain and blot carefully to remove excess liquid. 



9. Dehydrate with absolute alcohol; clear in xylol and mount 

 in cristalite or in Canada balsam. 



Results: 



Elementary bodies in cells associated with the following viruses 

 are stained scarlet : borna, zoster, rabies and pseudo rabies. 



ANILINE CRYSTAL VIOLET - GRAM'S IODINE 



For epithelial fibres 



Solutions required: 



A. AniUne crystal violet. 



B. Gram's iodine. 



C. Aniline xylol. 



Technique: 



1. Material should be fixed in absolute alcohol and embedded 

 in paraffin wax. 



2. Sections, not more than ^fi thick, are fixed to slides and 

 brought down to distilled water in the usual manner. 



3. Stain for ten to fifteen minutes in aniline crystal violet. 



4. Wash well in running water. 



5. Stain with Gram's iodine for ten to thirty seconds. 



6. Wash in water; drain; then blot carefully but thoroughly to 

 remove water. 



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