SECTION TWO 



7. Differentiate with aniline xylol, controlling at frequent inter- 

 vals by examination under the microscope. 



8. Wash well with xylol ; mount in balsam or D.P.X. 



Results: 

 Epithelial fibres are stained blue. 



ANILINE CRYSTAL VIOLET - LITHIUM CARMINE - 



IODINE 



For fibrin and for Gram-positive organisms in animal 



tissues 



Technique: 



1. Fix material in absolute alcohol, Camoy or alcohol-formalin, 

 and embed in paraffin wax. 



2. Fix sections to slides ; de-wax and pass through descending 

 grades of alcohol down to distilled water in the usual way. 



3. Stain in the lithium carmine solution for two to five minutes. 



4. Wash thoroughly in distilled water. 



5. Immerse in Solution D for five to ten minutes. 



63 



