MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



6. Rinse in distilled water; drain well and blot carefully. 



7. Cover with Gram's iodine solution and allow the stain to act 

 for five to ten minutes. 



8. Pour off the excess iodine solution and blot carefully with 

 filter paper. 



9. Differentiate with the aniline xylol solution until no more 

 purple coloration comes out. 



10. Drain, and blot carefully. 



1 1 . Rinse with several changes of xylol. 



12. Mount in balsam or in cristalite. 



Results: 



Fibrin and Gram-positive organisms are blue to blue-black 

 while nuclei are red. 



ANILINE BLUE - ORANGE G (Mallory) 



For collagenous and reticulin fibrils ; cartilage, bone, 

 amyloid, nuclei; fibroglia and elastin fibres 



Solutions required: 



A. Acid fuchsin J-i% aqueous. 



B. Aniline Blue - Orange G. 



Technique: 



Tissues are fixed in Zenker and embedded in paraffin wax, 

 Celloidin or L.V.N. 



1. Mount sections on slides and bring down to 90% alcohol; 

 then treat with iodine in the usual way to remove mercuric deposits. 



2. Bring down to distilled water and stain for one to ten minutes 

 in Solution A; then without washing: 



3. Stain for twenty minutes to one hour or longer in Aniline 

 Blue - Orange G ; then remove excess stain with several changes 

 of 95% alcohol. 



4. Dehydrate with absolute alcohol ; clear in xylol and mount in 



Cristalite. 



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