SECTION TWO 



Note: If Celloidin or L.V.N, sections are used the staining time 

 may be shortened and 95% alcohol should be used for decolor- 

 izing and dehydration ; terpineol for clearing. 



Results: 



Collagenous fibril, intense blue. Ground substances of cartilage, 

 bone, mucus, amyloid : varying shades of blue. Nuclei, myoglia, 

 neuroglia fibrils, axis cylinders, fibrin, nucleoli : red. Blood cor- 

 puscles and myelin: yellow. Elastic fibrils: pale pink or pale 

 yellow, or unstained; fibriloglia: red or unstained. 



Note: By omitting the acid fuchsin the collagenous fibres are 

 more sharply defined. 



AZAN STAIN 

 (Heidenhain) 



Solutions required: 



A. Azocarmine B .. .. 0-5 gm. 

 Distilled water . . . . 100 ml. 

 Glacial acetic acid . . . . i ml. 



Dissolve by warming ; cool and filter. 



B. q6% alcohol . . . . 100 ml. 



Dissolve by warming ; cool and filter. 



For staining, dilute i volume of this solu- 

 tion with 3 volumes of distilled water. 



6s 



