MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1 . Zenker-, Bouin- or Camoy-fixed tissues are stained from forty- 

 five to sixty minutes at 55° C. in Solution A; then at room tem- 

 perature for five to ten minutes. 



2. Wash in distilled water ; then differentiate in Solution B 

 until cytoplasm is pale pink, and nuclei are red and clear. 



3. Rinse for one half to one minute in Solution C. 



4. Transfer to Solution D for about one to three hours or 

 until the connective tissue is completely decolorized ; then wash 

 quickly in distilled water. 



5. Stain for one to two hours in the diluted Solution E, exam- 

 ining at ten- or fifteen-minute intervals to prevent over-staining. 



6. Wash quickly in distilled water; then differentiate in 95% 

 alcohol followed by absolute alcohol. 



7. Clear and mount. 



Results: 



Collagen stained deep blue ; reticulum, deep blue ; chromatin, 

 red; muscle tissue, reddish to orange; erythrocytes, red; neur- 

 oglia, reddish ; mucin, blue. 



AZO CARMINE - MALLORY STAIN 

 For Islets of Langerhans 



Solution required: 



A. Azocarmine, B, aqueous o-i% . . 100 ml. 

 Boil for about 5 minutes; then cool and add 

 2 ml. glacial acetic acid. Then warm to 60 °C. 

 and filter at that temperature. 



B. 90% alcohol 99 ml. -f- i nil. Aniline Oil. 



C. Iron alum 5% aqueous. 



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