MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



AZOCARMINE - HAEMATOXYLIN - ACID GREEN - 



ORANGE G 



For differential cell analysis of the rat anterior hypophysis 



Solutions: 



A. Zenker - Formol 



Potassium dichromate . . . . 25 gm. 



Mercuric chloride . . . . . . 50 gm. 



Ringer's solution (i.e. 0-9% saline) . . i litre. 



Add I mL neutral formaldehyde 40% solution per 10 ml. of the 

 above solution immediately before using. 



N.B. Use fresh stain for each batch of about twenty slides. 



Technique: 



1. Fix in solution A for six to twelve hours. 



2. Wash six to twelve hours in running water. 



3. Dehydrate by immersing for thirty minutes in each of the 

 following: 30%, 50%, 70%, 80% and 95% alcohol. 



4. Immerse in absolute alcohol, two changes, for one hour in 

 each. 



5. Immerse in a mixture consisting of equal parts of absolute 

 alcohol and cedarwood oil for one hour. 



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