MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



12. Differentiate in solution D (clove-toluol), controlling under 

 the microscope, for about ten to fifteen minutes. 



13. Rinse with two changes of toluol. 



14. Mount in permount-toluene or in Clearmount or Cristalite. 



Results: 



Zymogen granules, dark violet. Parietal cells, scarlet, and 

 nuclei, blue. The parietal cells are distinctly contrasted from the 

 pepsinogen cells. 



Reference: Cambel, P. and Sgouris, J. (1951), Stain Tech., 26, 243-6. 



BISMARK BROWN - METHYL GREEN 



For mucin, cartilage, and goblet cells in embryonic tissue, 



trachea and intestine 



Solutions required: 



A. Bismark brown 1% aqueous. 



B. Methyl green 0-5% aqueous. 



Technique: 



Tissues are fixed in Bouin or Zenker and embedded in paraffin 

 wax. 



1. Sections are brought down to distilled water; then stained 

 five to ten minutes in Solution A. 



2. Wash with 95% alcohol. 



3. Stain with Solution B until the preparation appears dark 

 green to the naked eye. 



4. Dehydrate with 95% and absolute alcohol; then clear in 

 xylol, and mount. 



Results: 



Cartilage: dark brown. Mucin: light brown. Nuclei of all 

 cells: green. 



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