SECTION TWO 



BIONDI - EHRLICH - HEmENHAIN STAIN 

 For chromatin, nucleoli, mucin, etc. 



Solution required: 



Biondi - Ehrlich - Heidenhain 0-9 gm. 

 Distilled water . . . . . . 100 ml. 



Dissolve by warming and stirring in a beaker. 

 When cool add : 



Chloroform . . . . . . 0-25 ml. 



Technique: 



1 . Fix tissues in saturated aqueous mercuric chloride and embed 

 in paraffin wax in the usual manner. 



2. Fix sections to slides; de-wax with xylol and pass through 

 absolute alcohol followed by 90% and 70% alcohol. 



3. Treat for the removal of mercuric precipitate by the standard 

 technique {see page 28). 



4. Immerse in the staining solution from six to twenty-four 

 hours. 



5. Rinse directly with 95% alcohol. 



6. Dehydrate with absolute alcohol. 



7. Clear in xylol and mount. 



Results: 



Chromatin is stained bluish green, while nucleoli are red; 

 mucin is stained green; erythrocytes, orange. Cytoplasm and 

 connective tissue elements are in varying shades of red. 



BEST'S CARMINE 

 For glycogen 



Note: This method has the advantage over the Lugol's iodine 

 technique in that fading does not occur so readily, and better 

 staining of glycogen is obtained. The disadvantages are that the 

 stain is less specific than in the iodine method, and the solution 



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