MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



3. Immerse in Solution C for half an hour at 37° C. agitating 

 at frequent intervals. 



4. Wash in several changes of distilled water. 



5. Immerse in Solution D for half an hour at 37° C. agitating 

 frequently. 



6. Wash in distilled water. 



7. Dehydrate by passing through ascending strengths of alcohol, 

 beginning with 70%, in the usual manner. 



8. Clear in xylol and mount. 



Results: 



Blood vessels are stained black against a background which is 

 almost colourless. 



BASIC FUCHSIN - METHYLENE BLUE 



N.B.: This solution must be freshly prepared 

 immediately before use. 



Technique: 



I. Pieces of fresh tissue, from the hippocampus major and cere- 

 bellum, not more than 3 mm. in thickness are fixed in Zenker, 

 washed, dehydrated, cleared, and embedded in paraffin wax. 



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