SECTION TWO 



2. Fix sections to slides ; de-wax with xylol. 



3. Pass through descending grades of alcohol and treat for the 

 removal of mercuric precipitate left by the fixative by the standard 

 technique. 



4. Place slides, section facing upwards, over the corner of a 

 tripod. 



5. Flood slide with the staining solution (Solution C, above) 

 and heat gently with a small bunsen flame for five minutes with 

 steam rising, taking care that the preparation does not catch fire. 



6. Allow the slide to cool for a few seconds ; then wash quickly 

 in water. 



7. Decolorize and diflFerentiate in 90% alcohol until the sec- 

 tions assume a faint violet colour. 



8. Rinse quickly in 95% alcohol. 



9. Dehydrate rapidly with absolute alcohol. 



10. Clear in xylol and mount. 



Results: 



Negri bodies are stained deep red, while the granular inclusions 

 are dark blue. Nucleoli are blue-black, while cytoplasm is bluish 

 violet, and erythrocytes appear copper coloured. 



BASIC FUCHSIN - GENTIAN VIOLET - IODINE 



For bacteria in sections 



Solutions required: 



A. Basic fuchsin . . . ,. . . 0-75 gm. 

 Alcohol absolute . . • • 30 ml. 

 Phenol crystals . . . . . . i gm. 



Distilled water . . . . . . 100 ml. 



B. Picric acid, saturated, aqueous. 



C. Aniline gentian violet. 



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