SECTION TWO 



Technique: 



1. Fix tissues in Zenker's Fluid for twenty-four hours; wash in 

 running water for two or three hours ; dehydrate ; clear ; embed 

 in paraffin wax in the usual manner. 



2. Sections, 4 to 5^^ in thickness, are stained from ten to thirty 

 minutes in Solution A ; then washed with distilled water. 



3. Stain with Methylene Blue (Loeffier) for fifteen to sixty 

 seconds ; then wash with water. 



4. Dehydrate and differentiate for a few seconds in absolute 

 alcohol; then clear in xylol and mount. 



Results: 



Negri bodies are stained crimson against a blue background. 



CARBOL FUCHSIN (Ziehl Neelsen) 

 For Nissl Bodies 



Solutions required: 



A. Carbol fuchsin (Ziehl Neelsen) . . 10 ml. 

 Acetic acid 0-5% . . . . 10 ml. 



B. Acetic acid 1% . . . . . . 10 ml. 



Formalin .. .. .. .. o-i ml. 



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