SECTION TWO 



CELESTIN BLUE - ORCEIN - LIGHT GREEN 



(Lendrum) 



For breast carcinoma and skin lesions 



(prepared with cold distilled water) 



Shake the dye with the iron alum solution in a 

 flask; then boil for three minutes. 

 Allow to cool ; filter ; then add 



Sulphuric acid, concentrated 

 Glycerine 



C. Eosin yellowish aqueous 1% 

 Gallic acid 0-5% aqueous 



D. Phosphomolybdic acid 1% aqueous. 



E. Masson^s Light Green: 

 Light green 2% aqueous . . 

 Glacial acetic acid .... 



Technique: 



1. Fix tissues in Zenker or Bouin; if the latter is used then 

 picric acid must be removed by washing de-waxed and dehydrated 

 sections on slides with saturated lithium carbonate solution. If 

 Zenker is employed ; then mercuric precipitate must be removed 

 after fixation by the standard technique. 



2. Wash; dehydrate; clear; embed in paraffin wax as usual. 



3. Fix sections to slides ; remove paraffin wax with xylol. 



4. Pass through descending grades of alcohol down to distilled 

 water in the usual manner. 



5. Stain for one half to two hours in Solution A, in a stoppered 

 grooved staining jar in the incubator or for twenty-four hours at 

 room temperature. 



89 



