MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



6. Rinse well with distilled water. 



7. Stain the nuclei with celestin blue (Solution B) for fifteen 

 minutes. 



8. Wash with running water for twenty minutes. 



9. Stain muscle and epidermis for two minutes with eosin 

 (Solution C). 



10. Decolorize the collagen somewhat in water or in 30% 

 alcohol. 



11. Immerse in phosphomolybdic acid for two minutes. 



12. Wash well in distilled water. 



13. Stain collagen with the light green solution. 



14. Dehydrate rapidly. 



15. Clear in xylol; mount in D.P.X. 



Results: 



Elastin, light brown. Nuclei, bluish purple. Muscle and epider- 

 mis, red. Collagen, green. 



From J. Path, and Bad., 40, 415-6, 1935 Lendrum, A. C. 

 " Celestin blue as a nuclear stain." 



CHLORAZOL BLACK 



A general-purpose stain, which can be used for whole tissues as 

 well as for sections. The stain requires no mordant or differenti- 

 ation, and it may be employed in aqueous or alcoholic solution. A 

 saturated solution in 70% alcohol stains ordinary sections in fif- 

 teen to thirty minutes ; the stain does not fade. 



The stain is particularly suitable for staining embryo ; kidney ; 

 intestine for demonstrating epithelial cells ; chromatin ; nucleoli ; 

 muscle fibres. 



Solution required: 

 Chlorazol Black, saturated in 70% alcohol. 



Technique: 



1. Tissues should be fixed in Zenker and embedded in paraffin 

 wax. 



2. Bring sections down to 70% alcohol and remove mercuric 

 precipitate in the usual manner. 



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