MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



6. Wash in running water for fifteen minutes ; then stain with 

 Ehrlich or Delafield haematoxylin for five to ten minutes. 



7. Wash in water; dehydrate in the usual manner; clear in 

 xylol and mount. 



Note: If Celloidin sections are used dehydration should be 

 carried out with Isopropyl alcohol in place of ethyl alcohol. 



Results: 



Amyloid, red; nuclei, blue. 



CONGO RED - ANILINE BLUE - ORANGE G 



For elastic fibres 



Solutions required: 



A. Aluminium chloride, 2% aqueous. 



B. Congo Red . . . . . . 2 gm. 



Sodium citrate . . . . . . 2-5 gm. 



Glycerin . . . . . . . . i ml. 



Distilled water . . . . • • 97 i^il- 



C. Aniline Blue, aqueous . . . . 1-5 gm. 



Orange G . . . . . . 2-25 gm. 



Resorcinol . . . . • • 3 gm- 



Phosphomolybdic acid 1% aqueous 100 ml. 



Technique: 



Tissues should be fixed in 10% formalin, and frozen sections 

 should be employed. 



1. Wash sections in water; then immerse them in Solution A 

 for ten minutes. 



2. Wash with water and drain; then stain in the Congo Red 

 solution for ten minutes. 



3. Wash with tap water ; then plunge the slide into a dish of tap 

 water and agitate it there for ten seconds. 



4. Wash again with tap water ; then stain from five to ten min- 

 utes in the Aniline Blue-Orange G solution (Solution C above). 



5. Rinse carefully in tap water; drain well and blot. 



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