SECTION TWO 



6. Dehydrate in absolute alcohol ; clear in origanum oil ; wash 

 in xylol and mount. 



Results: 

 Elastic fibres, bright red ; fibrin, dark blue. 



CONGO RED - EHRLICH HAEMATOXYLIN 

 For eleidin and keratohyalin 



Solutions required: 



A. Congo Red 0-05% aqueous. 



B. Ehrlich haematoxylin. 



Technique: 



1. Material should be fixed in absolute alcohol and embedded 

 in paraffin wax. 



2. Sections not more than 5^ thick are mounted on slides and 

 brought down to distilled water as usual. 



3. Stain for five to ten minutes in the Congo Red solution. 



4. Rinse in distilled water; then stain for five to ten minutes in 

 Ehrlich haematoxylin. 



5. Blue in tap water in the usual manner. 



6. Dehydrate; clear in xylol and mount. 



Results: 



Eleidin is stained red, while nuclei and keratohyalin are blue. 



CRESYLFAST VIOLET - TOLUIDINE BLUE - THIONIN 



(EHRLICH) 



A non-fading tri-basic stain for nerve cells and Nissl 

 granules, in normal and pathological tissues 



Solutions required: 



A. Cresylfast violet, CNS . . . . 2 gm. 



Toluidine blue . . . . . . i gm. 



Thionin (Ehrlich) 0-5 gm. 



Ethyl Alcohol 30% 200 ml. 



93 



