SECTION TWO 



Results: 



Elastic tissue stained blue-black ; smooth muscle, red ; collagen 

 green. 



EOSIN AZUR 2 - HAEMATOXYLIN (Maximow) 



For demonstration of inflammatory changes in haemo- 



poietic tissues 



Solutions required: 



A. Azur 2 eosin .. .. .. o-i gm. 



Distilled water . . . . . . loo ml. 



Heat to boiling point, then allow to cool. 



B. Solution A (as above) . . . . lo ml. 

 Distilled water . . . . . • 50 ml. 



C. Ehrlich haematoxylin. 



Technique: 



1 . Formalin-fixed material (sections or smears) are stained from 

 five to ten minutes with Ehrlich haematoxylin. 



2. Pour off excess stain ; immerse the preparation in tap water 

 until it appears blue to the naked eye ; then wash thoroughly with 

 distilled water and drain well. 



3. Stain from eighteen to twenty-four hours in Solution B (as 

 above) in a staining jar. If a staining jar is not available, place the 

 slide, resting face downwards, on two pieces of thin glass rod, so 

 that any precipitate formed is not deposited on the preparation. 



4. Differentiate in 95% alcohol until dense blue clouds cease 

 to come away from the preparation, and the red corpuscles and 

 collagen are pink. 



5. Immerse the preparation in three changes of absolute alcohol, 

 followed by two changes of xylol ; then mount. 



Results: 



Cartilage stained purple; basophil leucocytes and mast cell 

 granules, purple to violet; nuclei, blue; erythrocytes, pink; 

 cytoplasm, pink to blue ; eosinophil granules and secretion gran- 

 ules, pink. 



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