MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



ETHYL VIOLET - BIEBRICH SCARLET (Bowie) 

 For pepsinogen granules in gastric mucosa 



Solutions required: 



A. Stock solution. 



Ethyl violet-Biebrich scarlet i% 

 in 20% alcohol. 



B. Staining solution. 



Stock solution (as above) . . 0-5 ml. 



Alcohol 20% . . . . . . 100 ml. 



Technique: 



1. Tissues should be fixed in Regaud's fluid, washed in running 

 water, dehydrated, cleared and embedded in paraffin wax in the 

 usual manner. 



2. Fix sections to slides, dewax and pass through descending 

 grades of alcohol down to distilled water. 



3. Stain for twenty-four hours in Solution B in a covered 

 staining jar. 



4. Drain and wipe oflF excess liquid. 



5. Differentiate for about half an hour with a mixture con- 

 sisting of equal volumes of clove oil and xylol, controlling by 

 examination under the microscope at intervals. ^ 



6. Wash well with several changes of xylol ; mount in cristalite. 



Results: 



Pepsinogen of the pepsin-forming cells is stained violet, while 

 parietal cells are red. 



FEULGEN FUCHSIN 



For chromatin in animal cells 



Solutions required: 

 A. N/i hydrochloric acid. 



100 



