SECTION TWO 



B. Distilled water . . . . . . 200 ml. 



Boil; allow to cool to about 70° C; then add: 

 Basic fuchsin . . . . . . i gm. 



When dissolved, raise to boiling point ; then allow 

 to cool to 50° C. and add: 



2 ml. pure hydrochloric acid 



cone, and 

 2 gm. potass, metabisulphite. 



Allow to stand twenty-four hours then add i gm. 

 decolorizing carbon; shake well; filter. The solu- 

 tion if properly prepared will be colourless. 



C. Pure hydrochloric acid, cone. . . 2 ml. 

 Potass, metabisulphite . . . . 2 gm. 

 Distilled water . . . . . . 200 ml. 



D. Light green 0-25% in 90% alcohol. 



Technique: 



Tissues are fixed in Zenker or Helly for six to twelve hours ; then 

 washed in running water for twelve to twenty-four hours. They 

 should not be treated with iodine-alcohol. 



1. Sections are transferred from distilled water to ajar of Solu- 

 tion A at 60° C. for five to fifteen minutes; then rinsed in distilled 

 water. 



2. Transfer for one to one and a half hours to a jar of Solution 

 B at room temperature. 



3. Transfer for two to three minutes in each of three successive 

 lots of Solution C ; then wash thoroughly in distilled water. 



4. Counterstain for about two to five minutes in Solution D. 



5. Dehydrate; clear and mount. 



Results: 



Chromatin, purple. All other elements, transparent green. 



lOI 



