MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1. Paraffin sections are fixed to slides, de-waxed and taken down 

 through descending grades of alcohol to distilled water in the 

 usual manner. 



2. Stain for five to ten minutes with the haemalum solution, 

 examining under the microscope at intervals until a satisfactory 

 degree of staining has been achieved. 



3. Rinse for a few seconds in tap water. 



4. Stain for one or two seconds with the eosin solution. 



5. Rinse for a few minutes in running tap water. 



6. Pass through 70%, 90% and absolute, alcohol. 



7. Clear in xylol and mount. 



Results: 



Collagen, deep pink. Smooth muscle, pink. Cytoplasm, pale 

 pink. Nuclei, blue. 



HAEMATOXYLIN - AZOPHLOXINE 

 For muscle, connective tissue, ganglion cells, etc. 



Solutions required: 



A. Lavdowsky's Fixative: 



Formalin (40% formaldehyde) . . 10 ml. 



Glacial acetic acid . . . . . . 2 ml. 



Alcohol 95% . . . . . . 50 ml. 



Distilled water . . . . . . 40 ml. 



B. Haematoxylin (Delafield, Harris or 

 Ehrlich) 



C. Azophloxine . . . . . . 0*2 gm. 



Acetic acid 0*2% . . . . . . 100 ml. 



Add about i ml. chloroform as a preservative. 



D. Acetic acid 0-2% aqueous. 



E. Orange G. . . . . . . . . 2 gm. 



Phosphotungstic acid . . • • 4 gm. 



Distilled water . . . . . . 100 ml. 



Fast Green FCF . . . . . . 0-2 gm. 



no 



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