SECTION TWO 



2. Sections are brought down to distilled water; then stained 

 with Delafield haematoxylin for ten minutes. 



3. Wash and immerse in tap water for about five minutes until 

 the section appears blue to the naked eye. 



4. Wash rapidly with distilled water ; then stain for one to two 

 minutes with 1% eosin yellowish, aqueous. 



5. Wash quickly with distilled water; then dehydrate with 

 95% and absolute alcohol. 



6. Clear in xylol and mount. 



Results: 

 Nuclei are stained blue; cytoplasm, pink. 



HAEMATOXYLIN (Ehrlich) 

 For keratohyalin 



Solutions required: 



A. Haematoxylin (Ehrlich). 



B. Potass, permanganate o-i%. 



Technique: 



1. Material should be fixed in 10% formalin and embedded in 

 paraffin wax. 



2. Fix sections to slides and bring down to distilled water as 

 usual. 



3. Stain in Ehrlich haematoxylin for ten minutes. 



4. Pour off excess stain ; then rinse and blue in tap water, 



5. Immerse in potassium permanganate o-i% for ten seconds; 

 then wash well with water. 



6. Dehydrate; clear; mount in balsam. 



Results: 



Keratohyalin is stained blue-black while the other elements are 

 unstained or faintly stained. 



113 



