MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



HAEMATOXYLIN (Ehrlich) 

 For demonstrating sodium urate in animal tissue 



Solution required: 

 Ehrlich or Delafield haematoxylin. 



Technique: 



1 . Fix tissues in absolute alcohol and embed in Celloidin. 



2. Stain for five to ten minutes in the haematoxylin solution. 



3. Blue in saturated lithium carbonate solution. 



4. Rinse quickly in distilled water. 



5. Dehydrate with 95% alcohol. 



6. Clear in terpineol. 



7. Mount in balsam. 



Results: 



Sodium urate crystals and nuclei, deep blue. 



HAEMATOXYLIN (Ehrlich) - EOSIN 

 A general stain for animal tissues 



Solutions required: 



A. Haematoxylin (Ehrlich). 



B. Eosin, aqueous, yellowish 1% in tap water. 



Technique: 



1. Sections are mounted on slides and brought down to distilled 

 water in the usual manner, any mercurial deposit from the fixative 

 being removed by the standard technique. 



2. Stain from two to ten minutes in haematoxylin (Ehrlich) 

 depending upon the " ripeness " of the stain (a well-ripened 

 haematoxylin will act much more rapidly than a recently-prepared 

 solution). 



3. Rinse in water; then *' blue " in tap water; that is, immerse 

 the preparation in tap water from two to ten minutes or until the 

 preparation appears blue to the naked eye. 



4. The preparation should now be examined, while still wet, 



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