SECTION TWO 



Technique: 



1. Tissues may be fixed in io% formalin or in Zenker, if the 

 latter is used then mercuric precipitates must be removed from 

 the sections by the standard technique. 



2. Fix sections to slides ; de-wax with xylol and pass through 

 descending grades of alcohol to water in usual manner. 



3. Stain in the haematoxylin solution for five to twenty minutes. 



4. Rinse quickly in acid alcohol. 



5. Immerse in a large volume of tap water for two to five min- 

 utes. 



6. Stain in the aniline gentian violet for two to five minutes. 



7. Pour off excess stain, and without washing, blot the slide 

 carefully. 



8. Flood with Lugol's iodine and allow the solution to act for 

 two to five minutes. 



9. Pour off excess, and without washing, blot dry carefully. 



10. Decolorize for a few seconds with Solution D. 



11. Flood the preparation with erythrosin (Solution E, above) 

 and allow the stain to act from one half to one minute. 



12. Pour oflF excess stain and wash the preparation with Solution 

 D. 



13. Rinse well with xylol; drain off excess and blot dry care- 

 fully. 



14. Mount in balsam or Cristalite or Clearmount. 



Results: 



Nuclei, blue. Fibrin and Gram-positive organisms, purplish 

 blue. 



HAEMATOXYLIN 

 (Heidenhain) 



Solutions required: 



A. Iron alum 3 gm. 



Distilled water . . . . . . 100 ml 



Dissolve by shaking. 



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