MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



B. Haematoxylin io% in absolute 



alcohol (which has been ripened 



for three months or longer) . . 5 ml. 



Distilled water . . . . • • 95 r^l- 



C. Eosin yellowish 1% aqueous. 



Technique: 

 Fix in Zenker. Embed in paraffin wax. 



1. Sections are brought down to distilled water, then mor- 

 danted in Solution A for one half to three hours. 



2. Wash in tap water ; stain one to three hours in Solution B ; 

 then rinse in tap water. 



3. Differentiate in Solution A, controlling by examination under 

 the microscope. 



4. Wash in running water for five to ten minutes. 



5. Stain with Solution C for one to three minutes. 



6. Wash in tap water; dehydrate; then clear in xylol and 

 mount. 



Results: 



Nuclei are stained black; cytoplasmic structures, pink. 



HAEMATOXYLIN 

 For the identification of lipines 



Solutions required: 



A. Potassium bichromate 5% aqueous. 



B. Haematoxylin solution (Ehrlich). 



C. Potassium ferricyanide . . . . 2-5 gm. 



Borax . . . . . . . . 2 gm. 



Distilled water . . . . . . 100 ml. 



Technique: 



I. Tissues are fixed from twelve to twenty-four hours in 10% 

 formalin in normal saline. 



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