SECTION TWO 



2. Wash for several hours in running water. 



3. Make frozen sections and collect them in distilled water. 



4. Immerse in Solution A for twenty-four to forty-eight hours 

 at 37° C. 



5. Wash in several changes of distilled water, handling the sec- 

 tions with care (as they become brittle after immersion in Solution 



A). 



6. Immerse in Solution B for four to six hours at 37° C. 



7. Wash in distilled water. 



8. Differentiate in Solution C, controlling under the micro- 

 scope, until the ground cytoplasm is changed from black to yellow. 

 This process takes several hours. 



9. Wash thoroughly in five or six changes of distilled water; 

 then mount in glycerine jelly. 



Result: 



Lecithin and other lipines are stained black to deep blue (light 

 blue coloration should not be taken as positive). Lipides and 

 other tissue constituents are colourless. 



HAEMATOXYLIN (Kultschitzky) 



(Weigert's modification) 



For finer studies of cortical architecture and for total 



brain sections 



Solutions required: 



A. Weigerfs Secondary Mordant: 



Cupric acetate neutral, normal . . 5 gm. 

 Fluorchrome . . . . . . 2-5 gm. 



Distilled water . . . . . . 100 ml. 



Boil ; allow to cool ; then add : 

 Glacial acetic acid . . . . 5 ml. 



B. Haematoxylin (Kultschitzky). 



C. Lithium carbonate, saturated, 



aqueous . . . . . . 100 ml. 



Potassium ferricyanide 1% aqueous 10 ml. 



K 119 



