MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1. After fixing material in io% formalin mordant for four to 

 hwt days in Solution A. 



2. Dehydrate in ascending grades of alcohol in the usual way, 

 and embed in Celloidin. 



3. Immerse for twelve to twenty-four hours in the haematoxylin 

 (Solution B). 



4. Differentiate in Solution C from four to twelve hours, con- 

 trolling by examination under the microscope at intervals, and 

 changing the differentiating fluid three or four times. 



5. Wash thoroughly in distilled water. 



6. Dehydrate with 95% alcohol. 



7. Clear in terpineol. 



8. Drain well and blot carefully. 



9. Mount in balsam. 



Results: 



Finest myelin sheaths are stained a deep black. 



HAEMATOXYLIN - PHLOXINE - ANILINE 



GENTIAN VIOLET 



For actinomyces in sections 



Solutions required: 



A. Ehrlich haematoxylin. 



B. Phloxine 3% aqueous. 



C. Aniline gentian violet. 



D. Gram's iodine. 



Technique: 



1. Tissues are fixed in 10% formalin, washed, dehydrated, 

 cleared and embedded in paraffin wax in the usual manner. 



2. Fix sections to slides ; de-wax and bring down to distilled 

 water as usual. 



3. Stain with Ehrlich haematoxylin five to ten minutes; then 

 blue and wash in lithium carbonate saturated aqueous. 



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