MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



4. Wash with distilled water. 



5. Stain for five to ten minutes in a mixture consisting of one 

 volume of each of solutions A and B, and two volumes distilled 

 water. 



6. Wash for five minutes in running water. 



7. Stain for two to four minutes in Curtis Stain. 



8. Wash with 95% alcohol. 



9. Dehydrate with absolute alcohol. 



10. Clear in xylol and mount. 



Results: 



Chromatin, black. Cytoplasm, yellow. Collagen and fibres, red. 



Reference: Leach, E. H. (1946), Stain Tech., 21, 107-10. 



HAEMATOXYLESr - PONCEAU FUCHSIN - FAST 



GREEN FCF 



A micro-anatomical stain, superior to haematoxylin and 

 eosin for the differentiation of tissues in histo-pathological 

 work, superior to Van Gieson for collagen fibres. The 

 stain has also been found very valuable in cytological work 



Solutions required: 



A. Iron haematoxylin (Heidenhain) No. i. 



B. Iron haematoxylin (Heidenhain) No. 2. 



C. Picric acid 8% in 96% alcohol. 



D. Acid fuchsin 1% aqueous . . 100 ml. 

 Glacial acetic acid . . . . i ml. 



E. Ponceau de xylidine . . . . i gm. 



Distilled water . . . . . . 100 ml. 



Glacial acetic acid . . . . i ml. 



F. Phosphomolybdic acid 1% aqueous. 



G. Fast Green FCF 2% aqueous . . 100 ml. 

 Glacial acetic acid . . . . 2 ml. 



Technique: 



I. Bouin, formalin or Susa-fixed tissues are embedded and 



sectioned in the usual manner. 



m 

 124 



