MEDICAL AND BIOLOGICAL STAINING TECHNIQUES 



Technique: 



1. Tissues should be fixed in io% formalin and paraffin sections 

 employed. 



2. Stain for five minutes in a freshly prepared mixture consisting 

 of equal parts of Weigert's Haematoxylin A and B. 



3. Wash in tap water. 



4. Stain for three to five minutes in the acetic Ponceau, S 

 (Solution C). 



5. Rinse in distilled water. 



6. Stain for three to five minutes in the picro aniline blue 

 (Solution D). 



7. Wash for three or four minutes in 1% acetic acid solution. 



8. Dehydrate in ascending strengths of alcohol and clear in 

 xylol in the usual manner. 



9. Mount in acid balsam. 



Results: 



Connective tissue, glomerular basement membrane and reti- 

 culum, blue. Muscle and plasma, pink. Erythrocytes, bright red. 



HAEMATOXYLIN (Weigert) SCARLET R 



For demonstrating fatty acids crystals, soaps and neutral 



fats in fat necrosis 



Solutions required: 



A. Formalin 10% saturated with calcium salicylate. 



B. Copper acetate 10% aqueous. 



C. Weigert haematoxylin, A. 



D. Weigert haematoxylin, B. 



E. Borax 0-2% aqueous . . . . i litre 

 Potassium ferricyanide . . . . 2-5 gm. 



F. Scarlet R (Herzheimer). 



Acetone . . . . . . • • 50 ml. 



Alcohol, 70% . . . . . . 50 ml. 



Scarlet R . . . . . . . . i -5 gm. 



Heat on a hot water bath; then allow to cool 

 before filtering. 



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